1. Earliest experiment about gene regulation and expression study was studied by Francois Jacob and Jacques Monod in 1940s.
2. It's about syntheis of enzymes by Escherichia coli bacteria in a culture medium.
3. First, they cultured Escherichia coli in a medium containing glucose as respiratory substrate. Their growth is in a fast rate.
4. Then, the bacteria are transferred into a lactose medium, where lactose is the respiratory medium.
5. It is found that their growth rate is slow at first. But then, the rate resumes to normal after some time.
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